Purposes
The ECS project is directly connected to the understanding of agrosystems. The field experiments investigate grassland agrosystems as model. The research proposed herein addresses any kind of agrosystems.
We propose herein
To analyse the AM fungal community composition and dynamics colonising plants in the different plots to test the hypothesis of a decreased AM fungal community diversity when plant diversity is reduced. To do so, new molecular approaches will be used: from DNA & RNA extracts, a high throughput amplicon sequence analysis will be done using pyrosequencing.
To test the theory of embargo of C allocation to AM fungal cheaters tested under natural conditions using a SIP-RNA approach and controlled inoculations.
To better understand how the plant development strategy is impacted by the harboured endophytes. This will be done by the use of a particular experimental design started 2 years ago.
Fungal community composition will be studied in different experimental mixtures of prairial species differing in their growing strategy. Spatial distribution will be studied in relation with spatial colonisation of the plants in order to test that spots of microorganisms may be driven by plant clonal colonisation strategy. In particular plots, plant developmental response to competition will be correlated with their composition of endophytes, but also to root symbioses.
To understand unknown symbiotic interactions in roots by a substractive experimental SIPDNA & SIPRNA based approach.
We propose herein
To analyse the AM fungal community composition and dynamics colonising plants in the different plots to test the hypothesis of a decreased AM fungal community diversity when plant diversity is reduced. To do so, new molecular approaches will be used: from DNA & RNA extracts, a high throughput amplicon sequence analysis will be done using pyrosequencing.
To test the theory of embargo of C allocation to AM fungal cheaters tested under natural conditions using a SIP-RNA approach and controlled inoculations.
To better understand how the plant development strategy is impacted by the harboured endophytes. This will be done by the use of a particular experimental design started 2 years ago.
Fungal community composition will be studied in different experimental mixtures of prairial species differing in their growing strategy. Spatial distribution will be studied in relation with spatial colonisation of the plants in order to test that spots of microorganisms may be driven by plant clonal colonisation strategy. In particular plots, plant developmental response to competition will be correlated with their composition of endophytes, but also to root symbioses.
To understand unknown symbiotic interactions in roots by a substractive experimental SIPDNA & SIPRNA based approach.